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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins during Drosophila Oogenesis
doi: 10.3791/63778
Figure Lengend Snippet: Materials
Article Snippet: Drive the expression of Crag RNAi in the FE Forceps (Dumont 5) Fine Science Tools 11251–30 For dissection Glass Concavity Slide Electron Microscopy Sciences 7187804 For dissection (depression wells can be used instead) Goat anti-Rabbit IgG, Alexa Fluor 568 Invitrogen A11036 Secondary antibody (GM130 antibody) (5 μg/mL) Hoechst (Hoechst 33342) Invitrogen H3570 DNA Stain (1 ug/mL) Kimwipes Kimtech Fisher Scientific: 06–666 Delicate task wipers Leica Fluorescent Stereo Microscope M165 FC Leica For ovary imaging Microscope Slides Corning 294875X25 Microscope Slides Nutating platform rocker Corning Life Sciences 6720 For ovary fixation and staining Nutri-Fly BF Genesee Scientific 66–121 Fly Food Paraformaldehyde 20% Solution Electron Microscopy Sciences Fisher Scientific: 15713 For PFA 4% Phosphate Buffered Saline Tablets Fisher scientific BP2944100 For
Techniques: Staining, Concentration Assay, Dissection, Blocking Assay, Electron Microscopy, Expressing, Microscopy, Imaging, Saline, Super-Resolution Microscopy, Software